European leader in analytical sciences

       

High-resolution mass spectrometry of therapeutic proteins

Quality Assistance provides full characterisation of therapeutic proteins by high-resolution mass spectrometry (HRMS). Whether you are interested in monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), fusion proteins, small or large proteins, Quality Assistance has the tools to support you for the characterisation of drug substances and drug products, QC and stability studies. 

 

Our comprehensive GxP-compliant protein analysis workflow

  • Identity
    • Molecular weight at the intact and subunit levels
    • Peptide mapping (including deamidation and oxidation quantification)
    • Aggregates and fragments
    • Charge variants
    • Drug-to-antibody ratio of ADCs
    • Drug conjugation sites determination of ADCs
  • Glycosylation
    • N- and O-glycans profiling and relative quantification
    • N- and O-glycosylation sites determination
    • Sialylation profiling and quantification
  • Biosamples analysis (e.g. from preclinical and clinical studies)
    • Drug-to-antibody ratio of ADCs
    • Quantification 

 

Going further with your proteins 

Quality Assistance strives to provide its customers with tools to better understand analytical results. We offer two-dimensional LC/MS analysis to further analyse charge variants, fragments and aggregates, and ADCs isomery. Read more on our article featured in Current Trends in Mass Spectrometry.

Quality Assistance now offers state-of-the art hydrogen-deuterium exchange (HDX) analysis to learn more about proteins structures, stability and binding.

 

Quality Assistance offers you dedicated analysts and equipment 

  • 2 Waters Xevo G2-XS QTOF high-resolution mass spectrometers with two dedicated H-class Bio and one 2D-LC UPLCs for protein characterisation
  • An automated Waters HDX-2 hydrogen-deuterium exchange system
  • A Waters Xevo TQS mass spectrometer with a dedicated 2D-LC UPLC for protein quantification
  • A Bruker AmaZon ion-trap mass spectrometer with ETD capabilities
  • A microflex II LRF60 MALDI-TOF mass spectrometer
  • specialised, highly skilled scientists at the bench
  • Expert advice to determine a strategy that best suits your needs

 

Resources

Webinar

Webinar
webinar-quality-assistance

Mass Spectrometry toolkit for the characterisation of protein glycosylation
By Eric Largy, R&D Scientist

access-webinar-quality-assistance

Mass Spectrometry toolkit for the characterisation of ADCs 
By Dr Arnaud DELOBEL, R&D Director

access-webinar-quality-assistance

Webinar

Video

1D & 2D-LC/MS characterisation of monoclonal antibodies for QC and stability testing
By Dr Géry Van Vyncht, R&D Director

access-webinar-quality-assistance

Learn more about our partnership with Waters to offer our customers the best hardware and software solutions to their analytical challenges

access-webinar-quality-assistance

 

Read more about HRMS characterisation of proteins in our application notes:

close X

Recommend to a friend

Please complete the fields below