PCR (Polymerase Chain Reaction) is a method used to quantify nucleic acids. Whereas the classic quantitative PCR is based on an exponential amplification of target DNA by a DNA polymerase, ddPCR consists in dividing the PCR solution into ~ 20 000 highly uniform nanoliter-sized droplets per sample. The PCR reaction is therefore independently done in each of the droplets.
In comparison with quantitative PCR (qPCR), also available at Quality Assistance, the QX200™ droplet digital PCR system (from Bio-Rad) provides absolute quantification of target DNA or RNA molecules without the use of standard curves and is essential for viral vector analysis. Droplet digital PCR also allows for the analysis of complex samples without any extraction step and with less inhibitory effect. Finally, ddPCR shows a higher sensitivity and precision with a high throughput.
ddPCR equipment provides a solution for a wide range of applications, such as:
Detection of residual DNA (CHO, HEK, E.coli,...)
Determination of the viral vector genome integrity
Viral vector particle determination
Quantification of viral load
Distinction of genomic variations
Gene expression studies
Download our technical sheet to learn more about the equipment characteristics, specification and principle.