Quality Assistance contributes to Springer’s Mass Spectrometry of Glycoproteins
28th April 2021
The book Mass Spectrometry of Glycoproteins is now available on SpringerLink.com. This scientific review presents methods and protocols used for the analysis of glycoproteins at different levels and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. It was edited and partly written by Arnaud Delobel, R&D Director at Quality Assistance, in collaboration with 87 authors from 30 research laboratories all over the world.
Click here to access the publication
Quality Assistance's contribution:
Arnaud Delobel participated in the writing of three chapters including two with Claire Butré, Biochemistry & Characterisation Scientist at Quality Assistance. The published chapters present the methods and protocols developed by two of Quality Assistance's former scientists: Fabrice Cantais and Eric Largy.
Glycosylation of Therapeutic Proteins: A Critical Quality Attribute
Arnaud Delobel. Pages 1-21
Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS After Derivatization with Fluorescent Anthranilamide
Claire I. Butré, Eric Largy, Arnaud Delobel. Pages 179-188
Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS After Derivatization with Fluorescent Anthranilamide
Claire I. Butré, Eric Largy, Fabrice Cantais, Arnaud Delobel. Pages 237-247
About Mass Spectrometry of Glycoproteins:
The chapters in this book cover topics such as
- the role of glycosylation on the properties of therapeutic glycoproteins
- different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins
- mass spectrometry imaging methodology for glycosylation analysis in tissues
- approaches to characterizing glycosylation on cultured cells
- and the use of cloud computing to deploy mass spectrometry data analysis.