

Quality Assistance has proudly contributed to Elsevier’s publication Carbohydrate Analysis by Modern Liquid Phase Separation Techniques 2nd edition (978-0-12-821447-3).
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges.
This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques, as it
Covers the basic principles of modern liquid phase separation techniques, along with their applications
Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science
Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress
Arnaud Delobel, R&D Director, and Claire I. Butré, R&D Technical Leader, are the authors of Chapter 17 “HPLC- and CE-based methods for the characterization of therapeutic glycoproteins”
Introduction
Glycosylation of therapeutic proteins and structure/activity relationship
Analytical strategies for the characterization of therapeutic glycoproteins
Analysis of glycoproteins at the intact and subunit levels
Reversed-phase liquid chromatography
Hydrophilic-interaction liquid chromatography
Ion-exchange chromatography
Size-exclusion chromatography
Capillary gel electrophoresis
Capillary isoelectric focusing
Capillary-zone electrophoresis
Analysis of proteins at the glycopeptides level
Peptide mapping by reversed-phase liquid chromatography
Peptide mapping by hydrophilic-interaction liquid chromatography
Separation of glycopeptides on porous graphitized carbon (PGC)
Capillary electrophoresis
Analysis of glycoproteins at the glycan level
Strategies for glycan release and derivatization
P-LC/MS
PGC-LC/MS
HPAEC/PAD
Hydrophilic-interaction liquid chromatography
Mixed-mode chromatography
CE/LIF
Analysis of glycoproteins at the monosaccharide levels
Hydrolysis of glycoproteins into monosaccharides
HPAEC/PAD
RP-LC
CE/LIF