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Innovative ICP-MS/MS approach for the determination of phosphodiester to phosphorothioate ratio in phosphorothioate oligonucleot Quality Assistance Application Note

Innovative ICP-MS/MS approach for the determination of phosphodiester to phosphorothioate ratio in phosphorothioate oligonucleotides

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Phosphodiester to phosphorothioate ratio (P=O/P=S) is a critical quality attribute of phosphorothioate oligonucleotides. The determination of this ratio is commonly performed by P-NMR, which requires specific equipment and cannot be easily applied in a regulated (GxP) environment. For the first time, we present in this application note the preliminary results obtained for the development of an ICP-MS/MS method to determine P=O/P=S ratio.

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Authors
Juliusz Bianga
Caroline Cajot
Philippe De Raeve
Arnaud Delobel