Structural characterisation and epitope mapping by HDX-MS

Dr Arnaud Delobel
AATP, Romainville (France)

Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) is an innovative tool for the characterisation of biotherapeutics. Besides the study of higher-order structures, HDX-MS can also be used for epitope mapping studies in order to determine to which region of the target a monoclonal antibody binds. HDX-MS is a good alternative to techniques such as X-ray diffraction or NMR.