Characterisation of proteins by MS
Quality Asssistance provides a wide range of technologies to fully characterise therapeutic proteins.
Whether you are interested in monoclonal antibodies (mAbs), antibody-drug conjugates (ADCs), fusion proteins, small or large proteins, Quality Assistance has the tools to support you for the characterisation of drug substances and drug products, QC and stability studies.
![Characterisation of proteins by MS](/sites/default/files/2024-06/Quality-Assistance-Protein-characterisation.png)
Our comprehensive GxP-compliant protein analysis workflow
Identity
- Molecular weight at the intact and subunit levels
- Peptide mapping (including deamidation and oxidation quantification)
- Drug-to-antibody ratio of ADCs
- Drug conjugation sites determination of ADCs
Glycosylation
- N- and O-glycans profiling and relative quantification
- N- and O-glycosylation sites determination
- Sialylation profiling and quantification
Biosamples analysis (e.g. from preclinical and clinical studies)
- Drug-to-antibody ratio of ADCs
- Quantification
Going further with your proteins
Quality Assistance strives to provide its customers with tools to better understand analytical results. We offer two-dimensional LC/MS analysis to further analyse charge variants, fragments and aggregates, and ADCs isomery. Read more on our article featured in Current Trends in Mass Spectrometry.
Quality Assistance offers state-of-the art hydrogen-deuterium exchange (HDX) analysis to learn more about proteins structures, stability and binding (epitope mapping).
Quality Assistance offers you dedicated analysts and equipment
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3 Waters Xevo G2-XS QTOF high-resolution mass spectrometers with three dedicated H-class Bio and one 2D-LC UPLCs for protein characterisation
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An automated Waters HDX-2 hydrogen-deuterium exchange system
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1 Waters BioAccord mass spectrometer with a dedicaded I-Class UPLC
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A Bruker AmaZon ion-trap mass spectrometer with ETD capabilities
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An Autoflex MaX MALDI-TOF/TOF mass spectrometer
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5 specialised, highly skilled scientists at the bench
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Expert advice to determine a strategy that best suits your needs