Synthetic peptides vaccines
Synthetic peptide vaccines represent an important step forward in vaccinology, with applications ranging from infectious diseases to oncology. Their strengths lie in their targeted specificity, low toxicity and a favorable safety profile, setting them apart from traditional vaccines. By combining the simplicity of small chemical entities with the biological activity of complex therapeutics, they open new opportunities for innovative vaccine design.
Analytical challenges matched to complexity
This hybrid nature, however, often requires complex formulation to maximise efficacy. To enhance stability and boost immunogenicity, these peptides are frequently coupled to carrier proteins, linked to nanoparticles or combined with adjuvants such as alum.
These sophisticated formulations demand advanced analytical expertise, a field where we bring proven experience and fully integrated capabilities to ensure reliable characterisation and control of every component. Detecting chemical modifications, quantifying impurities, monitoring aggregation, characterising PEGylation and crucially, analysing the peptide-carrier linkage and the adsorption onto adjuvants, demand advanced methods tailored not only to the peptide's unique sequence and structure but also to the final multi-component formulation matrix. Our goal is to help you to demonstrate the safety, quality and efficacy of your synthetic peptide vaccines with total confidence.
At Quality Assistance, we aim to simplify complex challenges by building robust analytical strategies that deliver clear, reliable results, allowing you to confidently advance your synthetic peptide vaccines. Our approach ensures you meet all regulatory requirements, including those for complex and multi-component formulation, accelerating your product's path to market.
Specific analytical services for synthetic peptide vaccines
We adapt our analytical strategies to the specific challenges of peptide-based vaccines.
From early candidate selection through to batch release, we provide support designed to generate reliable, compliant data at every stage. This includes analytical assays tailored to the specific requirements of synthetic peptide vaccines, including formulation adjuvants:
| Purity profile | IEX / RP-(U)HPLC / icIEF |
|---|---|
| Counter-ion content (mass balance) | (U)HPLC (UV, MS) / ICP (OES, MS) |
| Mass distribution profile | MS (ESI-QTOF / MALDI-TOF) |
| Enantiomeric purity | (U)HPLC-UV / GC-MS / CE |
| Chemical modifications | (U)HPLC (UV, MS) / CE (UV, LIF) |
| Structural integrity | CD / FT-IR |
| Aggregation and particle size distribution | SEC-(U)HPLC (UV, RI, MALS) / AF4 / CE / DLS / SDS-PAGE / Imaging Particle Analysis |
| Disulphide bridges | SDS-PAGE / Peptide mapping (UPLC-UV/MS) / cGE |
| Free thiols | Fluorescence (NPM) / UV (Ellman, DTNB) |
| Degradation patterns (including Met oxidation, Asn deamidation) | IEX / RP-(U)HPLC / SE-(U)HPLC / SDS-PAGE / (U)HPLC(MS) / CE (icIEF, CE-SDS) |
| PEGylated peptides: Molecular weight distribution PEG-to-peptide ratio | MALDI-TOF / SEC (UV, RI, MALS) |
| PEGylated peptides: free PEG | HPLC / CAD |
| Assay | (U)HPLC (UV, Fluo, RI, MS) |
|---|---|
| Absolute protein content determination | ICP-MS (S, isotope dilution) |
| Determination of extinction coefficient | UV + ICP-MS (S, isotope dilution) |
| Immunoassays | ELISA / ECL (MSD) / Immunodiffusion (RID) / Luminex / SPR (Biacore) / Octet (BLI) / FACS |
| Drug / Metabolites | ELISA / ECL (MSD) / Luminex / UPLC (MS/MS) / ICP (OES, MS) / ESI-QTOF |
|---|---|
| Biomarkers | ELISA / ECL (MSD) / Luminex / Gyrolab / UPLC (MS/MS) |
| Vehicles / Buffers | ELISA / (U)HPLC (UV, RI, DAD, FLUO, ELSD, CAD, MS) / ICP (OES, MS) |
We cover all major vaccine technologies
Integrated services for vaccines
In addition to synthetic peptide vaccine analytics, we offer a full range of general services that apply to all vaccine categories, including batch release, stability studies testing and bioanalytical services.
Related services
Beyond vaccines
Synthetic peptide vaccines share many of the same analytical challenges as peptide-based therapeutics, from sequence confirmation to impurity profiling and bioanalysis in complex matrices. Explore our synthetic peptide page to see how our deep expertise bridges vaccine development and peptide therapeutics, ensuring consistency, regulatory compliance and confidence across all applications.
See our analytical services for synthetic peptide therapeutics
Ready to move forward ?
Developing synthetic peptide vaccines requires clarity and compliance. At Quality Assistance, we deliver the data you need to move forward with confidence. Get in touch with our experts to accelerate your vaccine project.