High Efficiency Protein A Affinity Columns for the LC-MS and 2D LC-MS Analysis of mAb and msAb
Stephan M. Koza (Waters Corporation)
Claire I. Butré
Arnaud Delobel
Jurgen Sans (Waters Corporation)
Stephen J. Shiner (Waters Corporation)
Beatrice Muriithi (Waters Corporation)
Chen Gao (Waters Corporation)
Matthew A. Lauber (Waters Corporation)

A quiver of LC-MS based approaches for the sub-unit and intact and analysis of intact monoclonal antibodies (mAb) and multispecific antibodies (msAb) directly from clarified cell-culture samples employing a newly developed high-efficiency (3.5 μm particles), high-pressure capable (6500 PSI/450 bar) 2.1 X 20 mm Protein A (ProA) affinity column