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Development of orthogonal chromatographic methods for the purity analysis of therapeutic oligonucleotides

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… Oligonucleotides have been in clinical development for approximately 30 years. Anti-sense oligonucleotides and aptamers were initially developed, … numerous clinical trials were performed and thousands of oligonucleotides underwent studies for a possible market …

Absolute Quantification of Oligonucleotides by ICP-MS/MS

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… an analytical method for the absolute quantification of oligonucleotides is a prerequisite. In this application … by triple quadrupole ICP-MS for the quantification of oligonucleotides in drug substances and drug products. The … the method was studied in terms of precision and accuracy. Oligonucleotides are a promising class of therapeutics, that …

Oligonucleotides: Absolute quantification and P=O/P=S in a single ICP-MS/MS run

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… digestion of the sample: accurate quantification of oligonucleotides can be performed using the fixed … Sodium may also be determined with the same method. … Oligonucleotides: Absolute quantification and P=O/P=S in a …

Oligonucleotides: A serious analytical challenge in terms of analytical characterisation

Article

… Oligonucleotides: A serious challenge in terms of analytical characterisation by Arnaud Delobel , R&D Director at Quality … a GMP/GLP environment. Though less complex than recombinant proteins, oligonucleotides represent a serious challenge in … available for the characterisation of peptide and protein drugs are constantly improving, less focus is put on …

OLIGONUCLEOTIDES: absolute quantification and P=O/P=S ratio in a single ICP-MS/MS run

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… is a critical quality attribute of phosphorothioate oligonucleotides. Quality Assistance developed an … by ICP-MS/MS to allow: Accurate quantification of oligonucleotides, without reference substance Easy … determine the P=O/P=S ratio in oligonucleotide samples. … OLIGONUCLEOTIDES: absolute quantification and P=O/P=S …

Oligonucleotides : absolute quantification and P=O/P=S ratio in a single ICP-MS/MS run

Resources

… is a critical quality attribute of phosphorothioate oligonucleotides. The determination of this ratio is … samples. The method could be used in routine testing of oligonucleotides in a GxP environment, as an orthogonal … be a solution for a fast and accurate generic method for oligonucleotides quantification without the reference oligo …

Innovative ICP-MS/MS approach for the determination of phosphodiester to phosphorothioate ratio in phosphorothioate oligonucleotides

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… is a critical quality attribute of phosphorothioate oligonucleotides. The determination of this ratio is … samples. The method could be used in routine testing of oligonucleotides in a GxP environment, as an orthogonal … is a critical quality attribute of phosphorothioate oligonucleotides. The determination of this ratio is …

Analysis of Oligonucleotides by Size-Exclusion Chromatography: SEC-UV, SEC-MS, SEC-MALS

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Analysis of Oligonucleotides by Size-Exclusion Chromatography: SEC-UV, SEC-MS, SEC-MALS

Resources

… The field of therapeutic oligonucleotides has seen remarkable progress over the last years . Oligonucleotides have emerged as promising candidates for … silencing, such as short interfering RNA (siRNA), antisense oligonucleotides (ASOs), microRNA (miRNA) and aptamers. …

Development of orthogonal chromatographic methods for the purity analysis of therapeutic oligonucleotides

Resources

… Oligonucleotides have been in clinical development for the … clear clinical benefit in rigorously controlled trials. Oligonucleotides are synthetised by a chemical process. … oligonucleotide of interest of a given length n but also oligonucleotides of unwanted length, such as n-1 …