Ion-Pair Reversed‑Phase LC method development for sgRNA impurity analysis - webinar hosted by Phenomenex
How do you reliably separate closely related impurities in long RNA molecules?
sgRNAs are central to CRISPR-Cas systems, but their analytical characterisation remains challenging. Small sequence variations and structural complexity require high-resolution methods capable of distinguishing closely related species such as N-1 truncations.
In this webinar, in collaboration with Phenomenex, Lara will share how we approached sgRNA impurity profiling using a structured, AQbD‑guided ion‑pair reversed‑phase LC strategy.
Why you should attend
You should attend this webinar if you:
- develop or optimise LC workflows for long RNA or sgRNA,
- face challenges separating closely related impurities such as N‑1 truncated species,
- need analytical results that are robust, transferable and suitable for routine use,
- want to move from empirical screening to structured, data‑driven analytical decisions.
How to attend
This webinar is offered in two live sessions to accommodate different time zones:
- Session 1 – May 19, 2026 - Americas time zones
- Session 2 – May 20, 2026 - Europe & India time zones
Application note
This approach is detailed in a joint application note developed with Phenomenex
Discover the application note
Speaker
- Lara Nercessian, R&D Scientist - Specialised in LC method development and impurity profiling for complex biomolecules, including nucleic acid modalities.