Mass Spectrometry Toolkit for the characterisation of ADCs

Arnaud Delobel, Scientific Manager

Access this 45 minute webinar to learn how state-of-the-art LC/MS technologies can be implemented for the efficient study of ADCs using analyses optimised for each level of information you need. 

Analytical performance of the different solutions proposed will be discussed, together with the presentation of numerous experimental results (with advantages and drawbacks) obtained during method development and optimisation.

We presented efficient and optimised approaches for the characterisation of ADCs by mass spectrometry, based on different levels of analysis: 

  • Intact protein for Drug-to-Antibody Ratio determination
  • Substructures (after IdeS / FabRICATOR digestion) 
  • 2D-LC/MS (HIC-RP) for DAR determination and identification of the different isoforms 
  • Peptide mapping for determination of conjugation sites and site-occupancy 

Applications of these methods to KADCYLA® and/or ADCETRIS® were also presented. 

The performances and limitations of the different analytical approaches were also discussed.