AAV purity and VP ratio determination by CE-SDS
AAV capsid protein purity and VP ratio are critical quality attributes in viral vector development. Their reliable measurement is key to ensuring batch consistency, supporting comparability studies and strengthening CMC strategies.
In this application note, we present an optimised CE-SDS method with laser-induced fluorescence (LIF) detection, developed to enhance peak resolution and robustness, under GxP conditions.
Download the full technical application note below ⬇️
Key highlights of the method
- LOQ established at 8 × 10⁹ vg/mL
- Clear separation of VP species
- Detection of low-level impurities
- 0.7% RSD (on purity determination) across two instruments and two days
How this supports your AAV programme?
Robust VP ratio determination supports:
- Confident batch-to-batch comparison
- Clear data interpretation during process optimisation
- Stronger comparability assessments
- Reliable CMC documentation
Reliable VP ratio assessment is essential for robust CMC and AAV development strategies.
Strengthen your AAV analytical strategy with a high‑performance CE‑SDS approach designed for robust viral vector characterisation.
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