Surface Plasmon Resonance

By
  • Fabian Vandermeers
  • Barbara Laborie
  • Manon Bruynbroeck
  • Florian Cambay
  • Arnaud Delobel
Date
Download file

Surface Plasmon Resonance (SPR) using Biacore technology enables precise, label‑free characterisation of biotherapeutics, delivering active concentration, affinity and kinetic data essential across development. This application note presents validated workflows including CFCA, Fc receptor interaction profiling, batch comparability, potency testing and influenza HA quantification.

Highlights

  • Calibration‑free active concentration determination
  • Kinetic and affinity profiling of adalimumab with Fcγ receptors and FcRn
  • Sensorgram‑based comparability assessments
  • ICH Q2‑validated potency assay for pembrolizumab
  • Accurate HA quantification across 0.0625–4 µg/mL

What does this mean for you ?

SPR brings consistency and decision‑driving data throughout development, from early screening to quality control. It ensures that critical attributes of biotherapeutics are quantified accurately and compared reliably across batches.

  • Consistent data across the development lifecycle
  • Robust support for stability studies and degradation monitoring
  • Confident batch‑to‑batch comparison through sensorgram analysis
  • Better understanding of antibody–receptor and antibody–target interactions
  • Validated potency and antigen quantification workflows for release testing

Download the full application note ⬇️

Download file

Complete this form and access this document in seconds.

Name
Address
Opt-in