Multi-attribute characterisation of adeno-associated viral vectors by dynamic and static multi-angle light scattering
Adeno-associated viral vectors are widely used in gene therapy, where consistent product quality relies on accurate characterisation of particle-related attributes. AAV samples may contain a heterogeneous mixture of species, including empty and full capsids, aggregates and other impurities that can affect their performance and safety.
This application note describes an analytical approach combining multi-angle dynamic light scattering (MADLS) and size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) to assess key critical quality attributes of AAV samples.
By integrating batch and separative light scattering techniques, this workflow provides complementary information on size, concentration, aggregation state and capsid content.
Supporting your AAV development and quality control
The combined MADLS and SEC-MALS workflow supports your decisions throughout your AAV development by providing orthogonal and complementary data on particle attributes. The workflow we developed:
- Supports the characterisation of particle size distribution and aggregation in both empty and full AAV samples
- Enables the absolute determination of capsid and genome-associated molar masses without reliance on reference standards
- Facilitates accurate determination of total particle titre, genome titre and full-to-empty ratios
- Improves comparability between samples by separating monomeric particles from aggregates prior to analysis
- Provides analytical data supporting the definition and monitoring of critical quality attributes
Together, these techniques contribute to a robust and transparent analytical strategy for AAV characterisation
Download the full application note to get a full overview of our analytical approach
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