Comprehensive immune profiling with four multiparametric flow cytometry panels

Multicolour flow cytometry enables high‑throughput, multiparametric phenotyping of PBMCs to support applications such as immune reconstitution monitoring, evaluation of immunomodulatory treatments and characterisation of immune alterations linked to infection, inflammation, autoimmunity or cancer.

This study presents a modular strategy built on four complementary panels, delivering both broad and high‑resolution immunophenotyping using the CytoFLEX LX flow cytomer (6 lasers, 23 parameters).

Download the full technical application note below ⬇️

Key highlights of the method

• Four complementary panels covering global profiling, T/NKT cells, B cells and innate immune subsets
• Optimised antibody titration using stain index calculations
• APD‑based compensation enabling matrix reuse across gain settings
• Standardised staining workflow with Fc blocking, FMO controls, viability and optional intracellular staining

How this supports your immune monitoring programme?

This modular strategy offers a robust framework for consistent and in‑depth immune characterisation:

• Repeatability and intermediate precision demonstrated using multiple vials from the same PBMC batch
• Accurate and biologically consistent measurements, matching the Immuno-Trol reference ranges
• Detailed identification of major and rare subsets, including memory, activation and maturation states
• Reduced variability through standardised workflows
• Improved comparability across analysts, days and PBMC batches

Applications

• Clinical immunomonitoring
• Monitoring of therapeutic responses
• Profiling immune signatures in disease contexts

Talk to our experts about your immunomonitoring strategy