Implementation and development of OCTET Red96e BLI platform

By
  • Thomas Henri
  • Florian Visée
  • Fabian Vandermeers
  • Arnaud Delobel
Date
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Bio-Layer Interferometry (BLI) provides a label-free approach for analysing interactions critical to biotherapeutic development. This application note demonstrates the implementation of the OCTET Red96e platform through case studies covering target binding, bispecific antibody analysis, Fcγ receptor interactions, FcRn characterisation, C1q binding and active concentration determination.

Key highlights

  • Affinity and kinetic characterisation across multiple biologically relevant interactions
  • Simultaneous evaluation of dual-target binding for bispecific antibodies
  • Demonstrated repeatability and intermediate precision for receptor-binding studies
  • Active concentration assay showing acceptable precision, accuracy and linearity

Applications

  • Monoclonal antibody target binding
  • Bispecific antibody characterisation
  • Fcγ receptor studies
  • FcRn analysis
  • C1q interaction assessment
  • Active concentration determination

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