2D UPLC/MS

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The 2D UPLC/MS system combines two chromatographic dimensions with mass spectrometry for advanced analytical workflows. It enables automated heartcutting and solvent exchange to make complex separations compatible with MS detection. This configuration supports detailed characterisation while maintaining full automation from injection to data acquisition.

System highlights

  • Online combination of two chromatographic columns hyphenated to mass spectrometry
  • Automated heartcutting with analysis of up to seven fractions
  • At-column dilution enabling desalting prior to MS analysis
  • Compatibility with first-dimension separations that are not MS-compatible
  • Fully automated workflow from first-dimension injection to MS data collection

Applications

The system is designed for a wide range of protein and biopharmaceutical analyses, using various chromatographic modes in the first dimension followed by RP/MS in the second dimension. Applications include:

  • Protein fragment and aggregate analysis
  • Charge variants analysis
  • ADC characterisation
  • Free drug quantification
  • On-line sample purification
  • On-line digestion for mAb subunit characterisation.

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