Digital Droplet PCR (ddPCR)

Droplet Digital PCR (ddPCR) provides absolute quantification of target DNA or RNA molecules without the use of standard curves and is essential for viral vector analysis. This approach delivers higher sensitivity, precision and reduced inhibitory effects compared with traditional quantitative PCR, also available at Quality Assistance. The system supports efficient workflows for complex samples and high‑throughput analysis.

ADVANTAGES

• Absolute quantification without standard curves
• High sensitivity and precision
• Reduced inhibitory effects and no extraction required for complex samples
• High‑throughput processing capabilities
• Uniform droplet generation using microfluidics
• Consistent plate sealing with a semi‑automated heat sealer
• Thermal gradient functionality for method optimisation
• Two‑colour optical detection for up to 96 samples per run

APPLICATIONS / USE CASES

ddPCR provides precise and sensitive measurement for multiple applications, including detection of residual DNA (CHO, HEK, E. coli), determination of viral vector genome integrity, viral vector particle determination, quantification of viral load, distinction of genomic variations, and gene expression studies.