ELISpot

The AID (Autoimmun Diagnostika) MultiSpot reader is a multifunctional imaging device designed for the analysis of enzymatic and fluorescent (FluoroSpot) based ELISpot assays.

• The device features a high-resolution CCD camera coupled with an automated microscope with multiple objectives, capable of being used with either 96 or 384-well plates.
• The unit is controlled with a 21 CFR part 11 compliant software.
• The optical design allows for the simultaneous measurement of multiple cytokine secretions at the single-cell level (FluoroSpot assay).
• The MultiSpot reader can be used in many research fields such as vaccine development, cancer, allergies, and immune cell characterisation.

ELISpot/FluoroSpot can address the following questions:

• Antigen-specific cytokine responses
• Antibody-specific secreting cells
• Immunomonitoring (vaccine immunogenicity)
• Cytotoxic T-cell activity (Granzyme B, IFN-y and Perforin released by T cells)