High Efficiency Protein A Affinity Columns for the LC-MS and 2D-LC-MS Analysis of mAb and msAb

By
  • Stephan M. Koza (Waters Corporation)
  • Claire I. Butré (Quality Assistance)
  • Arnaud Delobel (Quality Assistance)
  • Jurgen Sans (Waters Corporation)
  • Stephen J. Shiner (Waters Corporation)
  • Beatrice Muriithi (Waters Corporation)
  • Chen Gao (Waters Corporation)
  • Matthew A. Lauber (Waters Corporation)
Date
Location
PEGS 2026
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Monoclonal and multispecific antibodies require robust analytical strategies throughout development and manufacturing.

This poster highlights how a high-efficiency Protein A affinity column can be integrated into LC-MS workflows, enabling direct online analysis of antibodies from cell culture samples while reducing sample preparation.

The work demonstrates several complementary applications, including:

  • 2D-LC-MS subunit analysis of monoclonal and bispecific antibodies following online Protein A capture and automated reduction.
  • SEC-MS intact analysis for the assessment of antibody identity, aggregates, fragments, and other size variants.
  • Native Protein A-MS workflows for rapid intact mass analysis and antibody titer determination.
  • Characterisation of multispecific antibodies through the resolution of heavy-chain pairing variants.

By combining online Protein A purification with advanced LC-MS techniques, this approach enables rapid and information-rich characterisation of antibody titer, identity, size variants, and chain pairing directly from in-process samples.

Interested in learning more about advanced LC-MS characterisation of biotherapeutics?
Our experts can help you implement fit-for-purpose analytical workflows for monoclonal antibodies, bispecific antibodies and other complex biologics.

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