Characterisation of particle size distribution in Adeno-Associated Viral Vectors (AAVs) by Dynamic Light Scattering (DLS)

Adeno-associated viral vectors (AAVs) are widely used in the development of gene therapy approaches and require careful characterisation to ensure product quality and consistency. Particle size and particle size distribution within a sample are important attributes that can influence the understanding of AAV behaviour in solution. Dynamic Light Scattering (DLS) is a non-destructive analytical technique that enables the determination of particle size distribution for particles suspended in liquid.

This application note describes the development of a DLS method for AAV particle size determination and presents the evaluation of its analytical performance.

KEY HIGHLIGHTS

• Analysis performed directly on AAV samples without dilution optimisation requirements
• Detection of major particle populations and aggregates
• Stable measurements over 90 minutes at 20°C
• Robust performance between 15°C and 25°C
• Demonstrated repeatability and intermediate precision

HOW THIS SUPPORTS YOUR AAV CHARACTERISATION PROGRAMME?

The DLS approach described in this application note provides a practical method for assessing particle size distribution and monitoring sample characteristics

• Supports routine determination of AAV particle size distribution
• Enables the detection of multiple particle populations and potential aggregate formation within samples
• Provides information through both intensity-based and volume-based particle size distribution measurements
• Offers consistent performance through demonstrated precision and intermediate precision results