Analytical strategies for determining AAV capsid titre: a comparative study

By
  • Paula Paci
  • Thomas Henri
  • Florian Visée
  • Fabian Vandermeers
  • Damien Mouvet
  • Arnaud Delobel
Date
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This application note compares ELISA, Gyrolab, SPR and BLI for AAV capsid titre determination. All methods were qualified for AAV2 and AAV9, demonstrating reliable accuracy and precision across defined concentration ranges.

Value for gene therapy development

This comparative study provides a practical framework to support robust and informed selection of analytical methods for AAV capsid titre determination. It:

  • Supports accurate capsid titre measurement, a critical parameter for dosing and safety.
  • Enables selection of fit-for-purpose methods based on sensitivity, throughput and development stage.
  • Facilitates cross-platform comparability using orthogonal analytical approaches.
  • Strengthens analytical robustness in regulated and GxP-compliant environments.
  • Supports troubleshooting and optimisation at low viral particle concentrations.

Together, these results demonstrate how complementary analytical technologies can reinforce AAV analytical strategies across development and manufacturing.

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