Characterisation of Humira Adalimumab by high resolution mass spectrometry

Due to their mode of production, monoclonal antibodies are complex and heterogeneous molecules.

Their analysis usually requires the use of many orthogonal analytical techniques in order to fully characterise the different variants. In this application note, the use of mass spectrometry as a powerful tool for adalimumab characterisation is presented.

Mass spectrometry is a technique that is now widely used for biopharmaceuticals characterisation. Thanks to innovations in both hardware and software, this technique can now be used routinely. We present in this application note the use of mass spectrometry to characterise Humira® at different levels.