Characterisation of the glycosylation of Enbrel: Etanercept by high resolution mass spectrometry

Due to their mode of production, therapeutic glycoproteins present complex and heterogeneous glycosylations.

Their analysis usually requires the use of many orthogonal analytical techniques in order to fully characterise their nature and location. In this application note, the use of mass spectrometry as a powerful tool for Etanercept glycosylation characterisation is presented.