Evaluation of TCR-pMHC affinity and its implications for T cell responsiveness

Interactions between the T cell receptor (TCR) and peptide-major histocompatibility complexes (pMHCs) play a central role in T cell activation and adaptive immune responses. Understanding the affinity and kinetics of these interactions is essential for TCR engineering, immunotherapy development and immune monitoring applications. In this application note, we compare Surface Plasmon Resonance (SPR) and Bio Layer Interferometry (BLI) approaches to characterise the interaction between an affinity-enhanced NY-ESO-1-specific TCR and its target pMHC complex. The study provides practical insights into assay design, technology selection and affinity assessment strategies.

Key highlights of the method

• Comparative evaluation of SPR and BLI technologies for TCR-pMHC characterisation
• Assessment of affinity and kinetic parameters for an affinity-enhanced NY-ESO-1-specific TCR
• Evaluation of different assay formats and immobilisation strategies
• Assessment of assay specificity and analytical performance

Applications

• TCR engineering programmes
• Cancer immunotherapy research
• TCR-pMHC interaction studies
• Affinity and kinetics characterisation

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